Sequencing-based Virus Hunting and Virus Detection

Virus hunting.

Viral diagnosis and discovery are receiving increasing emphasis with the recognition of their importance in addressing the challenges of emerging infectious and chronic diseases, and the advent of antiviral drugs with which to reduce the morbidity and mortality of viral infections. Here we review the status of the field including the use of molecular, proteomic and immunological assays for vira...

Detection of Herpes Simplex Virus Antibodies Using the Whole Virus and Recombinant gD

Background and Aims: Herpes simplex virus type 1 (HSV1) remains a potentially serious health problem world wide. All infected people, including asymptomatic ones, are potential sources for virus transmission. Virus envelope contains at least 13 glycoproteins, which glycoprotein D is the major target of immune responses. The aim of this study was development of a specific method that is a more...

Serological and Molecular Detection of Akabane Virus in Iran

The present study was conducted on sixty healthy female domestic ruminants that suffered from still births or abortion, to find sero-positive cases and also to determine the probable role of uterine tissue in diagnosis of akabane viral infection and viral persistency. Because of this, a serological test (ELISA) and molecular (RT-PCR) diagnostic methods were used to find the seropositive cases a...

"Virus hunting"using radial distance weighted discrimination

Motivated by the challenge of using DNA-seq data to identify viruses in human blood samples, we propose a novel classification algorithm called " Radial Distance Weighted Discrimination " (or Radial DWD). This classifier is designed for binary classification, assuming one class is surrounded by the other class in very diverse radial directions, which is seen to be typical for our virus detectio...

Comparison between Micro and Tube Virus Neutralization Tests for Detection of Serological Responses to Infectious Bursal Disease Virus Following Vaccination